*Etiologic Risk Group (see link below): Risk Group 3 (RG3)
*Disease Information (sort of like the Intro to your Mini Research Write up):
Tuberculosis (TB) is an airbone spread illness that typically affects the respiratory system. Over 95% of TB deaths occur in low- and middle-income countries, and it is among the top 5 causes of death for women aged 15 to 44. Symptoms of active TB include a prolonged cough (3 or more weeks), chest pain, coughing up blood, weakness, weight less, loss of appetite, chills, fever, and night sweats. Alternatively, latent TB shows and feels no symptoms. Tuberculosis can be identified by a skin test or TB blood test. Treatment includes taking several drugs for 6 to 12 months with strict guidelines.
Essentiality of this protein: Rv2419c has essentiality data Gene/Ortholog: Tb927.5.3580 (OG4_12291); Phenotype: no significant loss or gain of fitness in bloodstream forms (3 days); Source study: alsford
Gene/Ortholog: Tb927.5.3580 (OG4_12291); Phenotype: no significant loss or gain of fitness in bloodstream forms (6 days); Source study: alsford
Gene/Ortholog: Tb927.5.3580 (OG4_12291); Phenotype: significant gain of fitness in procyclic forms; Source study: alsford
Gene/Ortholog: Tb927.5.3580 (OG4_12291); Phenotype: no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford
--- List cost and quantity of substrate reagents, supplier, and catalog #
β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate
Cost/Quantity: $314.50/100mgSupplier: Sigma-Aldrich Catalog #: N1161, N6005, and N8129 Structure (PDB or Homology model) PDB ID: 2A6P
Current Inhibitors: Metavanadate Expression Information (has it been expressed in bacterial cells): Expressed in E. coli cells
Purification Method: recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-R3-pRARE2 cells by nickel affinity chromatography and gel filtration
Image of protein (PyMol with features delineated and shown separately):
Description: a6p_bio_r_500.jpg
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): MSSWHAARTVVRATGLGCRRAQSVGMGVRNHRLLLLRHGETAWSTLGRHTGGTEVELTDTGRTQAELAGQLLGELELDDPIVICSPRRRTLDTAKLAGLTVNEVTGLLAEWDYGSYEGTTPQIRESEPDWLVWTHGCPAGESVAQVNDRADSAVALALEHMSSRDVLFVSHGHFSRAVI TRWVQLPLAEGSRFAMPTASIGICGFEHGVRQLAVLGLTGHPQPIAAG
*length of your protein in Amino Acids:228
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website: 24.5888 kDa
Molar Extinction coefficient of your protein at 280 nm wavelength: 36230 Lmol-1cm-1
*Target (protein/gene name):glucosyl-3-phosphoglycerate phosphatase
*NCBI Gene # or RefSeq#:83332
*Protein ID (NP or XP #) or Wolbachia#: NP_216935.1
*Organism (including strain): Mycobacterium tuberculosis H37Rv
*Etiologic Risk Group (see link below): Risk Group 3 (RG3)
*Disease Information (sort of like the Intro to your Mini Research Write up):
Tuberculosis (TB) is an airbone spread illness that typically affects the respiratory system. Over 95% of TB deaths occur in low- and middle-income countries, and it is among the top 5 causes of death for women aged 15 to 44. Symptoms of active TB include a prolonged cough (3 or more weeks), chest pain, coughing up blood, weakness, weight less, loss of appetite, chills, fever, and night sweats. Alternatively, latent TB shows and feels no symptoms. Tuberculosis can be identified by a skin test or TB blood test. Treatment includes taking several drugs for 6 to 12 months with strict guidelines.
Link to TDR Targets page (if present): http://www.tdrtargets.org/targets/view?gene_id=8530
Link to Gene Database page: http://www.ncbi.nlm.nih.gov/gene/885727#genomic-context
Essentiality of this protein:
Rv2419c has essentiality data
Gene/Ortholog: Tb927.5.3580 (OG4_12291); Phenotype: no significant loss or gain of fitness in bloodstream forms (3 days); Source study: alsford
Gene/Ortholog: Tb927.5.3580 (OG4_12291); Phenotype: no significant loss or gain of fitness in bloodstream forms (6 days); Source study: alsford
Gene/Ortholog: Tb927.5.3580 (OG4_12291); Phenotype: significant gain of fitness in procyclic forms; Source study: alsford
Gene/Ortholog: Tb927.5.3580 (OG4_12291); Phenotype: no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms; Source study: alsford
Is it a monomer or multimer as biological unit? (make prediction athttp://www.ebi.ac.uk/msd-srv/prot_int/pistart.html):
Monomer
Complex of proteins?: MGLP, GPG, and GG
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
No orthologous druggable targets
*EC#: 5.4.2.1
Link to BRENDA EC# page: http://www.brenda-enzymes.org/enzyme.php?ecno=5.4.2.1
-- Show screenshot of BRENDA enzyme mechanism schematic
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay (see Sigma links below)http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Enzyme_Assay/phosphoglycmutase.pdf
-- -or link (or citation) to paper that contains assay informationN/A
-- links to assay reagents (substrates) pages.
http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/2/n8129pis.pdf
--- List cost and quantity of substrate reagents, supplier, and catalog #
β-Nicotinamide adenine dinucleotide, reduced disodium salt hydrate
Cost/Quantity: $314.50/100mg Supplier: Sigma-Aldrich
Catalog #: N1161, N6005, and N8129
Structure (PDB or Homology model)
PDB ID: 2A6P
Current Inhibitors: Metavanadate
Expression Information (has it been expressed in bacterial cells): Expressed in E. coli cells
Purification Method: recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-R3-pRARE2 cells by nickel affinity chromatography and gel filtration
Image of protein (PyMol with features delineated and shown separately):
*Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
MSSWHAARTVVRATGLGCRRAQSVGMGVRNHRLLLLRHGETAWSTLGRHTGGTEVELTDTGRTQAELAGQLLGELELDDPIVICSPRRRTLDTAKLAGLTVNEVTGLLAEWDYGSYEGTTPQIRESEPDWLVWTHGCPAGESVAQVNDRADSAVALALEHMSSRDVLFVSHGHFSRAVI TRWVQLPLAEGSRFAMPTASIGICGFEHGVRQLAVLGLTGHPQPIAAG
*length of your protein in Amino Acids:228
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website: 24.5888 kDa
Molar Extinction coefficient of your protein at 280 nm wavelength: 36230 Lmol-1cm-1
TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html). Input your amino acid sequence to it.
CTAGAGGACATCGCTGGAGACCTGCGCCGAAGCATTCCACACATCAAGGCGCCACCGGATGCTCTCGAAGTCGGAGCCCGGGGCCCAATGGCCACTCAGCTGAGTCCAACTGGCATTGCCCATGCCGCCCAAAGCCGGCCAGTTGGCCACCGGCAACTTCAGCAGCGCCGCCGACAACGCGGCGATCAGACCCCCATGGGCTACCAGCACCACCGGGCGATCCGGCTCGTCAGCGCCACCCCATTCCGGTTCGCTGGCAACCAACTCGGCAACCAACGGCCGACTTCGGGCAGCCACGTCAACCCTGCTTTCCCCGCCGTGCGGCGCCCAGGTCGCATCCTCGCGCCAGGCCAACCGGGCGCCCGGGGCATCAGCGTCGATCTGAGCGTGGGTTAAGCCCTGCCAATCGCCAAGGTGAGTTTCCCGCAATCGGGTGTCGACCCGGACCACAAGGCCGGTGCGCTCGCCCAGCTTGACCGCCGTGTCATATGCGCGGCGCAGGTCCGACGATACGATCAGTAGCGGCTGCCGCTTGCCCAGCACCTCGGCGGCCGCGACCGCTTGGGTGCGGCCAAGTTCGCTCAACTCAGTGTCCAGCTGGCCCTGCATCCGGCTACCGACGTTGTAGTC CGTTTGTCCATGCCGCAGCATCACCAGTCGCCGCGCTCTCAT
*GC% Content for gene:
66.815476190476
*CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):
CTG GAG GAT ATC GCG GGA GAT CTC CGG CGC AGC ATC CCG CAC ATC AAG GCG CCG CCG GAC GCC TTG GAG GTC GGC GCC CGC GGT CCG ATG GCG ACC CAA TTG TCG CCG ACC GGC ATC GCG CAC GCG GCC CAG TCG CGG CCC GTG GGT CAC CGC CAG TTG CAG CAG CGC CGC CGC CAG CGG GGC GAC CAG ACC CCG ATG GGT TAC CAA CAT CAC CGC GCG ATC CGG CTG GTC TCC GCC ACC CCA TTC CGC TTC GCC GGT AAT CAA CTC GGT AAC CAG CGT CCC ACG TCC GGC TCG CAC GTC AAT CCG GCG TTC CCG GCG GTG CGT CGG CCG GGC CGG ATC CTG GCC CCA GGC CAA CCG GGC GCC CGG GGC ATC TCG GTG GAT CTG TCG GTC GGT TAG GCC CTC CCG ATC GCG AAA GTC TCG TTC CCG CAG TCC GGA GTC GAC CCG GAC CAC AAA GCC GGC GCC CTG GCG CAG CTG GAC CGC CGG GTC ATT TGT GCG GCG CAG GTC CGC CGG TAT GAC CAG TGA CGG CTG CCC TTG GCC CAA CAC CTC GGC GGG CGC GAT CGC CTG GGG GCC GCC AAG TTC GCC CAA CTG TCC GTG CAG TTG GCC TTG CAC CCG GCG ACC GAC GTG GTG GTG CGC CTG AGC ATG CCG CAG CAC CAC CAA AGC CCA CGT TCG CAC
*GC% Content for gene (codon optimized):
77.3 %